siRNA / miRNA gene silencing Rat Schwann cells

Get tips on using Live/Dead Cell Double Staining Kit to perform Live / Dead assay mammalian cells - HUVEC

Get tips on using Live-Dead Cell Staining Kit (BioVision) to perform Live / Dead assay mammalian cells - HUVEC

Get tips on using Pierce™ Cell Surface Protein Isolation Kit to perform Protein isolation Mammalian cells - HUVEC

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Microglia Medium to perform Stem cell Differentiation media hiPSCs differentiation into Microglial-like cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary porcine primary airway epithelial cell

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media PLC/PRF/5 cells-spheres

Get tips on using RPMI-1640 Medium to perform Stem cell culture media hLung Mesenchymal Stem Cells (MSCs)

Get tips on using Adipogenic Induction Medium to perform Stem cell Differentiation media mPericytes differentiation into adipogenic cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human blood endothelial cell