Protein Expression Eukaryotic cells HEK293

Get tips on using pCX-puro-mouFSH to perform Protein Expression Eukaryotic cells - CHO mouse FSH

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using pPIC-xyn11B to perform Protein Expression Eukaryotic cells - P. pastoris Penicillium oxalicum xyn11B

Get tips on using pPIC-xyn11A to perform Protein Expression Eukaryotic cells - P. pastoris Penicillium oxalicum xyn11A

Get tips on using pPIC-xyn10B to perform Protein Expression Eukaryotic cells - P. pastoris Penicillium oxalicum xyn10B

Get tips on using pPIC-xyn10A to perform Protein Expression Eukaryotic cells - P. pastoris Penicillium oxalicum xyn10A

Get tips on using pPIC9K-anPhyA to perform Protein Expression Eukaryotic cells - P. pastoris Aspergillus niger PhyA

Get tips on using pPIC9/MoL to perform Protein Expression Eukaryotic cells - P. pastoris M. oleifera lectin

Get tips on using BacPAK6-ΔChi/Cath to perform Protein Expression Eukaryotic cells - S. frugiperda β-gal

Get tips on using pYES2-αAI-OPT to perform Protein Expression Eukaryotic cells - S. cerevisiae αAI-OPT