rna-isolation-purification-cells-primary-rat-cortical-neurons

Get tips on using Ni-NTA Superflow 96 BioRobot Kit (4) to perform Protein tag Purification of His-tagged proteins

Get tips on using RosetteSep™ Human Monocyte Enrichment Cocktail to perform Cell Isolation Monocyte

Get tips on using EasySep™ Human Monocyte Enrichment Kit to perform Cell Isolation Monocyte

Get tips on using pgMAX system-rabbit voltage-dependent calcium channel β2a subunit to perform Protein Expression Prokaryotic cells - E. coli rabbit voltage-dependent calcium channel β2a subunit

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation E. coli DH5α

Get tips on using Laemmli Lysis-buffer to perform Protein isolation Bacteria - Clostridium difficile

Get tips on using Laemmli Lysis-buffer to perform Protein isolation Bacteria - Bordetella pertussis

Get tips on using QIAprep Spin Miniprep Kit to perform Plasmid Isolation Bordetella avium

Get tips on using QIAprep Spin Miniprep Kit to perform Plasmid Isolation Brucella spp