ELISA (kit) Human Serum Cytokine measurements (Multiplex assay)

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Annexin V-FITC Apoptosis Kit to perform Necrosis MIA PaCa-2

Get tips on using Apoptosis and Necrosis Quantification Kit to perform Necrosis MIA PaCa-2

Get tips on using EpiTect 96 Bisulfite Kit (2) to perform Bisulfite DNA Modification Fluids

Get tips on using FastLane Cell cDNA Kit (50) to perform cDNA synthesis Cell lines

Get tips on using HiSpeed Plasmid Midi Kit (25) to perform Plasmid Isolation E. coli

Get tips on using QIAxcel DNA Screening Kit (2400) to perform Analysis of DNA fragments

Get tips on using DNeasy PowerLyzer PowerSoil Kit (100) to perform DNA isolation / purification Insects

Get tips on using REPLI-g Mini Kit (100) to perform Whole Genome Amplification Parasites

Get tips on using EZQ™ Protein Quantitation Kit to perform Protein quantification Fluorimetric method