siRNA / miRNA gene silencing Human MCF-7

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Get tips on using FITC Annexin V Apoptosis Detection Kit I (RUO) to perform Apoptosis assay cell type - SMMC-7721, HEPG2

Products BD Biosciences FITC Annexin V Apoptosis Detection Kit I (RUO)

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - Cancer cell lines Liver cancer cell lines Hepato cellular carcenoma (SMMC-7721, Huh7 & HepG2))

Products Qiagen RNeasy Mini Kit

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell culture media Choroid plexus-like tissue generation from SFEBq

Products Thermo Fisher Scientific Gibco™ DMEM/F-12, GlutaMAX™ supplement

Get tips on using pPIC9K-mdFshβ to perform Protein Expression Eukaryotic cells - P. pastoris mdFshβ

Products Finn-Arne Weltzien, Department of Basic Sciences and Aquatic Med pPIC9K-mdFshβ

Get tips on using EmbryoMax MEF Conditioned Media to perform Stem cell culture media Mouse trophoblast stem cells

Products Sigma-Aldrich EmbryoMax MEF Conditioned Media

Get tips on using EmbryoMax MEF Conditioned Media to perform Stem cell culture media hESC lines H9, H1

Products Sigma-Aldrich EmbryoMax MEF Conditioned Media

Get tips on using mRFP-green fluorescent protein (GFP)-LC3 adenoviral vector to perform Autophagy assay cell type - Macrophages

Products HanBio Technology mRFP-green fluorescent protein (GFP)-LC3 adenoviral vector

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.

RNA RNA isolation / purification Bacteria Gram negative Pseudomonas aeruginosa

Generally isolating RNA from Gram-negative bacteria is easy, however keeping your working environment clean and RNase free (use RNase inhibitor) is essential. Some common points to keep in mind: a) Use fresh samples for isolation or store them by freezing in RNA stabilizing buffer until use. b) Choose the bacterial input amounts carefully, to ensure buffer volumes are adequate and not to overload the columns.

RNA RNA isolation / purification Bacteria Gram negative Escherichia coli

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

RNA RNA isolation / purification Cells immortalized CHO-K1

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