rna-isolation-purification-cells-immortalized-b35

Get tips on using QuantiPro™ BCA Assay Kit to perform Protein quantification Mammalian cells - RAW264.7

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Human - Precision cut lung slices Target preparation kit (RNA Amplification + Hybridization + control)

Get tips on using pDDGFP2_A2AR to perform Protein Expression Eukaryotic cells - S. cerevisiae A2AR

Get tips on using pSynInsCPOT to perform Protein Expression Eukaryotic cells - S. cerevisiae Insulin

Get tips on using pSynInsPOT to perform Protein Expression Eukaryotic cells - S. cerevisiae Insulin

Get tips on using pSynAmyCPOT to perform Protein Expression Eukaryotic cells - S. cerevisiae Amylase

Get tips on using pSynAmyPOT to perform Protein Expression Eukaryotic cells - S. cerevisiae Amylase

Get tips on using pHBM146 to perform Protein Expression Eukaryotic cells - S. cerevisiae XynHB

Get tips on using pPpT4_CalB to perform Protein Expression Eukaryotic cells - P. pastoris Lipase