rna-isolation-purification-cells-immortalized-mda-mb-361

Get tips on using SensiFAST™ Probe No-ROX One-Step Kit to perform RNA quantification qPCR - fibroblast

Get tips on using STEMdiff™ Trilineage Differentiation Kit to perform Stem cell Differentiation media Differentiation of Human primed induced pluripotent stem cells (UMN PCBC16iPS) into naive pluripotent stem cells

Get tips on using CTCF (D31H2) XP® Rabbit mAb #3418 to perform ChIP Anti-bodies CTCF

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray RNA amplification & Labeling - Mouse brain tissue Biotin

Get tips on using miRCURY LNA™ microRNA Power Labeling Kits to perform Microarray RNA amplification & Labeling - Rat spinal cord Hy5

Get tips on using pVT-opti-mdr3 to perform Protein Expression Eukaryotic cells - P. pastoris Pgp

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - K562 cells

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using Glut1 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)

Get tips on using CD74 siRNA and shRNA Plasmids (h) to perform RNA sequencing Human - HT-1376 (urinary bladder cell line)