Protein isolation Mammalian cells

Get tips on using E.Z.N.A.® Plasmid Mini Kit II to perform Plasmid Isolation Shigella sonnei

Get tips on using E.Z.N.A.®Yeast Plasmid Mini Kit to perform Plasmid Isolation S. cerevisiae

Get tips on using PureLink™ HiPure Plasmid Maxiprep Kit to perform Plasmid Isolation DH10Bac (Bacmid)

Get tips on using DNA-spin™ Plasmid DNA Purification Kit to perform Plasmid Isolation Enterobacteriaceae

Get tips on using RosetteSep™ HLA B Cell Enrichment Cocktail to perform Cell Isolation HLA B Cell

Get tips on using EasySep™ HLA B Cell Enrichment Kit to perform Cell Isolation HLA B Cell

Get tips on using EasySep™ HLA T Cell Enrichment Kit to perform Cell Isolation HLA T Cell

Get tips on using RosetteSep™ HLA T Cell Enrichment Cocktail to perform Cell Isolation HLA T Cell

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.