ChIP acH3 Bovine Donkey

Reporter gene assays are designed to test the regulation of the expression of a gene of interest. This is usually done by linking the promoter of the gene of interest with a gene such as a firefly luciferase, which can be easily detected by addition of luciferin that leads to an enzymatic reaction to produce luminescence. The enzymatic reaction can be correlated to the expression of the gene of interest. Another luciferase gene that can be used is Renilla luciferase. For an appropriate luciferase assay: 1. the reporter should express uniformly in all cells, 2. specifically respond to effectors that the assay intends to monitor, 3. have low intrinsic stability to quickly reflect transcriptional dynamics. It is important to have an equal number of cells plated in each testing condition to avoid any incorrect readouts. Reporter assays could be single or dual reporter assays. The reporter could be both luciferases. Most dual-luciferase assays involve adding two reagents to each sample and measuring luminescence following each addition. Adding the first reagent activates the first luciferase reporter reaction; adding the second reagent extinguishes first luciferase reporter activity and initiates the second luciferase reaction. Dual-luciferase assays have some advantages, including 1. reduces variability, 2. reduces background, 3. normalizes differences in transfection efficiencies between samples.

Get tips on using p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 to perform Western blotting p21

Get tips on using Bone DNA Purification Kit to perform DNA isolation / purification Tissue - bone

Get tips on using CD20 V450 to perform Flow cytometry Anti-bodies Human - CD20

Get tips on using CD122 antibody | TU27 to perform Flow cytometry Anti-bodies Human - CD122

Get tips on using CD206 antibody | MR5D3 to perform Flow cytometry Anti-bodies Mouse - CD206

Get tips on using CD204 antibody | 2F8 to perform Flow cytometry Anti-bodies Mouse - CD204

Get tips on using Anti-CCSP - Rabbit to perform Flow cytometry Anti-bodies Mouse - CCSP

Get tips on using CD43 antibody | DFT-1 to perform Flow cytometry Anti-bodies Human - CD43

Get tips on using CD38 Monoclonal Antibody (HIT2) to perform Flow cytometry Anti-bodies Human - CD38