Site Directed Mutagenesis (SDM) Human Deletion PC-3

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Human - Endometrial stromal cells Target preparation kit (Amplification + Hybridization + control)

Get tips on using SQSTM1 Antibody (D-3) to perform Cell cytotoxicity / Proliferation assay cell type - K562

Get tips on using GeneChip® HT 3' IVT PLUS Reagent Kit to perform Microarray Human - Precision cut lung slices Target preparation kit (RNA Amplification + Hybridization + control)

Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 3 µm to perform Cell migration / Invasion cell type - isolated human neutrophils

Get tips on using Rac1/2/3 Antibody #2465 to perform Western blotting Rac1

Get tips on using Active/Pro-Caspase 3 Monoclonal Antibody (31A1067) to perform Western blotting Caspase-3

Get tips on using Recombinant Anti-Caspase-3 antibody [E87] (ab32351) to perform Western blotting Caspase-3

A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.

Get tips on using pSUPER.retro.neo+gfp vector- Syn G (exon 3) siRNA to perform shRNA gene silencing Mouse - RGC-5 Syn G (Exon 3)

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Deletion STING exon 5