CRISPR Hamster Deletion CHO-K1

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human coronary artery smooth muscle cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary bovine coronary artery smooth muscle cells

Get tips on using pET-21a(+) DNA to perform Protein expression and purification Bacteria - Escherichia coli Prefoldin (PFD)

Get tips on using pQE-80L vector to perform Protein expression and purification Bacteria - Escherichia coli IFABP-Aβ

Get tips on using CelLytic™ B Plus Kit to perform Protein isolation Bacteria - Escherichia coli

Get tips on using QIAamp Fast DNA Tissue Kit to perform DNA isolation / purification Tissue - colon

Get tips on using AllPrep DNA/RNA Mini Kit to perform DNA isolation / purification Tissue - colon

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Colorectal aenocarenoma (SW48) - paraffin embeded

Stem cells have the unique ability to self-renew or differentiate themselves into various cell types in response to appropriate signals. These cells are especially important for tissue repair, regeneration, replacement, or in the case of hematopoietic stem cells (HSCs) to differentiate into various myeloid populations. Appropriate signals refer to the growth factor supplements or cytokines that mediate differentiation of various stem cells into the required differentiated form. For instance, HSCs can be differentiated into dendritic cells (with IL-4 and GM-CSF), macrophages (with m-CSF) and MDSCs (with IL-6 and GM-CSF). Human pluripotent stem cells (hPSCs) and induced pluripotent stem cells (iPSCs) can be first cultured in neural differentiation media (GSK3𝛃-i, TGF𝛃-i, AMPK-i, hLIF) to form neural rosettes, which can be differentiated into neural or glial progenitors (finally differentiated into oligodendrocytes). Neural progenitors can be finally differentiated into glutaminergic (dibytyryl cAMP, ascorbic acid) and dopaminergic (SHH, FGF-8, BDNF, GDNF, TGF-𝛃3) neurons. Thus, it is important to first identify the self-renewing cell line: its source and its final differentiation state, followed by the supplements and cytokines required for the differentiation, and final use. Timelines are another thing that is considered. For instance, it takes 7-10 days to form neural rosettes from iPSCs and 3 days to differentiate neural progenitors to neurons. Finally, the stability for stem cell culture media varies. It is advised to make fresh media every time when differentiating HSCs to myeloid populations, whereas neural differentiation media may remain stable for two weeks when stored in dark between 2-8C.

Get tips on using QIAprep 96 Turbo Miniprep Kit (24) to perform Plasmid Isolation E. coli