Protein expression and purification Mammalian cells HEK 293

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using QuantiNova Pathogen +IC Kit (500) to perform PCR Conventional / Qualitative PCR - mammalian DNA

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Get tips on using EZ DNA Methylation-Gold Kit to perform PCR Methylation specific PCR - Mammalian DNA

Get tips on using Hot Start Taq DNA Polymerase to perform PCR Hot start PCR - Mammalian DNA

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Get tips on using FastStart™ Taq DNA Polymerase to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using Taq PCR Master Mix Kit to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using Platinum™ Taq DNA Polymerase to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using Ion AmpliSeq™ Library Kit 2.0 to perform PCR Multiplex PCR - Mammalian DNA