Protein Expression Prokaryotic cells E. coli

Hello Iam a phd student in pharmacy and i want to know if this technology is suitable to knockout or silencing part of the gas5 gene in BV2 cells please

Get tips on using EpiQuik Chromatin Immunoprecipitation (ChIP) Kit to perform ChIP Rat - Colon

Get tips on using Anti-Collagen I antibody [COL-1] (ab90395) to perform Western blotting Type I collagen

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.

Get tips on using Collagen I antibody to perform Western blotting Type I collagen

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Colorectal aenocarenoma (SW48) - paraffin embeded

Get tips on using Anti-Collagen III antibody (ab7778) to perform Western blotting Type III collagen

Get tips on using Anti-Type I Collagen to perform Immunohistochemistry Collagen Type I - Goat Mouse -NA-

Get tips on using Anti-Collagen, Type VII to perform Immunohistochemistry Collagen Type VII - Rabbit Human -NA-

Get tips on using Goat Anti-Type III Collagen to perform Immunohistochemistry Collagen Type I - Goat Mouse -NA-