siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)

Get tips on using PerCP/Cyanine5.5 anti-human CD49d Antibody to perform Flow cytometry Anti-bodies Human - CD49d

Get tips on using PerCP/Cyanine5.5 anti-human CD4 Antibody to perform Flow cytometry Anti-bodies Human - CD4

Get tips on using CD11b Antibody, anti-human/mouse, FITC to perform Flow cytometry Anti-bodies Human - CD11b

Get tips on using APC anti-human CD90 (Thy1) Antibody to perform Flow cytometry Anti-bodies Human - CD90

Get tips on using APC-H7 Mouse Anti-Human CD44 to perform Flow cytometry Anti-bodies Human - CD44

Get tips on using CD133/2 Antibody, anti-human, APC to perform Flow cytometry Anti-bodies Human - CD133

Get tips on using CD133/2 Antibody, anti-human, PE to perform Flow cytometry Anti-bodies Human - CD133

Get tips on using Human EGFR In-Cell ELISA Kit (ab126419) to perform ELISA Human - EGFR

Get tips on using RNAqueous®-Micro Total RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized CAMA-1

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.