siRNA / miRNA gene silencing Human NCL-H40

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using FastDigest NcoI to perform Restriction Enzymes NcoI

Get tips on using NcoI R6513 to perform Restriction Enzymes NcoI

Get tips on using NcoI-HF® to perform Restriction Enzymes NcoI

Get tips on using NcoI restriction enzyme to perform Restriction Enzymes NcoI

Get tips on using NcoI NEB#R0193 to perform Restriction Enzymes NcoI

Get tips on using NcoI (10 U/µL) to perform Restriction Enzymes NcoI

Get tips on using pET30 Ek/LIC-Colα3(IV) NC1 to perform Protein Expression Prokaryotic cells - E. coli Colα3(IV) NC1

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - H4

Get tips on using Anti-ATG4B antibody produced in rabbit to perform Autophagy assay cell type - H4