siRNA / miRNA gene silencing Mouse MLO‐Y4

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using pPIC9K-Msp to perform Protein Expression Eukaryotic cells - P. pastoris myostatin propeptide

Get tips on using Human Myeloperoxidase Quantikine ELISA Kit to perform ELISA Human - MPO

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Myocytes

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media Human myogenic progenitor cells

Get tips on using TeSR™-E8™ to perform Stem cell Differentiation media hPSC or hiPSCS differentiation into myogenic cells

Get tips on using Gibco™ MEM α, Nucleosides to perform Stem cell Differentiation media hMSCs differentiation into myogenic cells

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media hMSCs differentiation into myogenic cells

Get tips on using MYH9 CRISPR/Cas9 KO Plasmid (h) to perform CRISPR Rat - Deletion PC12 myosin IIA (Myh9)

Get tips on using Gibco™Ham's F-12K (Kaighn's) Medium to perform Stem cell culture media Human myogenic progenitor cells