CRISPR Mouse Deletion RMA cells

Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media iPSCs or hESCs differentiation into Neuronal cells

Get tips on using DeadEnd™ Fluorometric TUNEL System to perform TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells

Get tips on using TUNEL Assay Kit - BrdU-Red to perform TUNEL assay cell type - A127, U87MG, U251MG, T98G human glioblastoma cells

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Human primary breast ephitelial cells-organoids

Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell culture media Human Dental pulp stem cells (hDPSC)

Get tips on using Gibco™DMEM, low glucose, pyruvate to perform Stem cell culture media Human Tendon Stem/Pluripotence cells (TSPCs)

Get tips on using Gibco™Essential 8™ Medium to perform Stem cell Differentiation media hiPSC differentiation into Human Neuronal cells

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Immortalized cell lines MDA-MB-231

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.