shRNA gene silencing Human HEK 293T

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Passage number Discussion

HeLa cells I used are passage 40. Are these cells good to perform Cell cycle analysis on?

Discussions Passage number
DMEM/F-12 Product

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media hESC-derived brain organoids

Products Thermo Fisher Scientific DMEM/F-12
EMBacY#5 Product

Get tips on using EMBacY#5 to perform Protein Expression Eukaryotic cells - Hi5 Soluble G protein (Hendra Virus)

Products Sabine Suppmann, Max-Planck Institute of Biochemistry EMBacY#5
mTeSR™1 Product

Get tips on using mTeSR™1 to perform Stem cell culture media hESC lines H9, H1

Products STEMCELL technologies mTeSR™1

Get tips on using Gibco™IMDM to perform Stem cell Differentiation media hESCs differentiation into SFEBq

Products Thermo Fisher Scientific Gibco™IMDM

Get tips on using COLUMBIA BLOOD AGAR BASE to perform Bacterial cell culture media Helicobacter pylori

Products Thermo Fisher Scientific COLUMBIA BLOOD AGAR BASE

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Mouse Heart

Products Qiagen RNeasy Plus Mini Kit

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Tissue - Rat Heart

Products Molecular Research Center, Inc. TRI Reagent® MRC

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - immortalized HeLa

Products Qiagen RNeasy Plus Mini Kit

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

Products Qiagen miRNeasy FFPE Kit

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