Flow cytometry Anti-bodies Mouse

Get tips on using CD96 (TACTILE) Monoclonal Antibody (NK92.39), PE, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD96

Get tips on using CD206 (MMR) Monoclonal Antibody (19.2), PE, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD206

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using B7-H4 Monoclonal Antibody (188), PE, eBioscience™ to perform Flow cytometry Anti-bodies Human - B7-H4

Get tips on using CD33 Monoclonal Antibody (WM-53 (WM53)), PE, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD33

Get tips on using CD206 (MMR) Monoclonal Antibody (19.2), PE-Cyanine7, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD206

Get tips on using CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), eBioscience™ to perform Flow cytometry Anti-bodies Human - CD163

Get tips on using FOXP3 Monoclonal Antibody (PCH101), Alexa Fluor 700, eBioscience™ to perform Flow cytometry Anti-bodies Human - FOXP3

Get tips on using CD4 Monoclonal Antibody (OKT4 (OKT-4)), FITC, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD4