Flowcytometry CD3

Get tips on using miScript II RT Kit (50) to perform cDNA synthesis Tissue

Get tips on using SuperScript® II Reverse Transcriptase to perform cDNA synthesis Tissue

Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Mouse - CD4+ T

Get tips on using SuperScript IV VILO Master Mix to perform cDNA synthesis Cell lines

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human CD14+ monocytes

Get tips on using SuperScript® II Reverse Transcriptase to perform cDNA synthesis Cell lines

Get tips on using RevertAid RT Reverse Transcription Kit to perform cDNA synthesis Cell lines

Get tips on using p27 Antibody (F-8): sc-1641 to perform Western blotting CDKN1B

Get tips on using ImProm-II™ Reverse Transcription System to perform cDNA synthesis Tissue

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.