Protein Expression Eukaryotic cells HeLa R19

Get tips on using pMmEG(TA)-rhBMP-4 to perform Protein Expression Eukaryotic cells - CHO BMP-4

Get tips on using pIEX-5-6His-mMBP-UMODpXR to perform Protein Expression Eukaryotic cells - HEK293 mMBP

Get tips on using p1.2-Hygro-FSH-B-chain to perform Protein Expression Eukaryotic cells - CHO FSH

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using pFastBac-MRP4-his6 to perform Protein Expression Eukaryotic cells - S. frugiperda human MRP4-his6

Get tips on using pPICZαC-MRP4-his6 to perform Protein Expression Eukaryotic cells - P. pastoris human MRP4-his6

Get tips on using pUC19/Pr-EC to perform Protein Expression Eukaryotic cells - K. lactis α-l-Fucosidase

Get tips on using pPIC9-His-FIP-Ganoderma to perform Protein Expression Eukaryotic cells - P. pastoris Ganoderma FIP