protein-expression-and-purification-mammalian-cells-hek-293-mt-pa

Get tips on using QuantiNova SYBR Green PCR Kit (2500) to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using Gibco™Ham's F-12 Nutrient Mix to perform Mammalian cell culture media PC-3

Get tips on using FGMTM-3 Cardiac Fibroblast Growth Medium-3 BulletKit to perform Mammalian cell culture media HCF

Get tips on using McCoy's 5A Medium, ATCC® 30-2007™ to perform Mammalian cell culture media A253

Get tips on using Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System to perform Reporter gene assay β-galactosidase substrates - U87 and U251 glioblastoma cells

Get tips on using - to perform Protein quantification

DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.