Get tips on using Low Range DNA Ruler Plus to perform DNA Ladder Low Range
Get tips on using Low Molecular Weight DNA Ladder to perform DNA Ladder Low Range
Get tips on using Fluorescent Low Range DNA Ladder to perform DNA Ladder Low Range
Get tips on using Fluorescent Long Range DNA Ladder to perform DNA Ladder Long Range
Get tips on using Long Range DNA Ladder (linear scale) to perform DNA Ladder Long Range
Get tips on using TriDye™ Ultra Low Range DNA Ladder to perform DNA Ladder Low Range
Get tips on using E-Gel™ Ultra Low Range DNA Ladder to perform DNA Ladder Low Range
Get tips on using E-Gel™ Low Range Quantitative DNA Ladder to perform DNA Ladder Low Range
I have extracted RNA from brain tissue but my RNA concentrations are as low as 5ng/ml with my highest being around 80ng/ml. Will I be able to perform cDNA conversion with concentrations as low as these?
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment