siRNA / miRNA gene silencing Human 501 Mel and SK Mel 28 FANCD2

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media Glioma differentiation into Human Neuronal lineage

Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media Glioma differentiation into Human Neuronal lineage

Get tips on using STEMdiff™ Definitive Endoderm Kit to perform Stem cell Differentiation media Differentiation of Human hESCs into Endoderm

Get tips on using pCW-LIC to perform Protein expression and purification Bacteria - Escherichia coli Fbxo7

Get tips on using STEMdiff™ Cerebral Organoid Maturation Kit to perform Stem cell culture media Brain organoids from Human iPSCs

Get tips on using Gibco™DMEM, low glucose, pyruvate to perform Stem cell Differentiation media Glioma differentiation Human astrocytic lineage

Get tips on using Gibco™DMEM, high glucose, pyruvate to perform Stem cell Differentiation media Glioma differentiation Human astrocytic lineage

Get tips on using MagNA Pure Compact RNA Isolation Kit to perform RNA isolation / purification Cells - primary human mesenchymal stem cells

Get tips on using Multiplex CRISPR/Cas9 Assembly System Kit to perform CRISPR Human - Activation hATCB

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.