Get tips on using pDG101 to perform Protein Expression Prokaryotic cells - E. coli mScarlet-I
Get tips on using pZH713 to perform Protein Expression Prokaryotic cells - E. coli PP7cp-SYFP2
Get tips on using pGETS118 to perform Protein Expression Prokaryotic cells - B. subtilis cellulosomal complexes
Get tips on using pHYαCGT7 to perform Protein Expression Prokaryotic cells - B. subtilis α-CGTase
Get tips on using pHYαCGT1 to perform Protein Expression Prokaryotic cells - B. subtilis β-CGTase
Get tips on using pAF3 to perform Protein Expression Prokaryotic cells - B. subtilis PHY US417
Get tips on using Cell Proliferation Kit I (MTT) to perform
Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - A549
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
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