siRNA / miRNA gene silencing Human RMS

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using gRNA_Cloning Vector to perform CRISPR Mouse - Deletion RMA cells Trh4

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized RMA-S

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized RMA-S

Get tips on using BECN1 Antibody (H-300) to perform Autophagy assay cell type - rMC-1

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - rMC-1

Get tips on using LC3B (D11) XP® Rabbit mAb (Biotinylated) to perform Autophagy assay cell type - rMC-1