ELISA (kit) Human Serum Cytokine measurements (Multiplex assay)

Get tips on using RNeasy Protect Bacteria Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Enterococcus faecalis

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram positive Streptococcus pneumoniae

Get tips on using GeneJET Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram positive Streptomyces. Sp

Get tips on using DNA-free™ DNA Removal Kit to perform

Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform

Get tips on using RiboPure™ RNA Purification Kit, bacteria to perform

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using MinElute Reaction Cleanup Kit (250) to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using MinElute Gel Extraction Kit (250) to perform DNA gel extraction / PCR product purification Product size < 15Kb

Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Cells - primary bovine aortic endothelial cells