ELISA (kit) Human Serum Cytokine measurements (Multiplex assay) -NA-

Get tips on using ApoAlert™ DNA Fragmentation Assay Kit to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Get tips on using NucleoSpin® RNA Blood to perform RNA isolation / purification Tissue - Human Blood / Serum / Plasma / Buffy coat

Get tips on using Cellular DNA Fragmentation ELISA to perform DNA Damage Assay Saos-2

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using Autophagy Assay Kit to perform Autophagy assay cell type - A549

Get tips on using Cell Cycle Assay Kit (Fluorometric - Green) to perform DNA quantification Human - Hep G2

Get tips on using TransAM® ER Transcription Factor ELISA Kits to perform ELISA Human - Estrogen receptor (ESRs)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Autophagy Assay Kit to perform Autophagy assay cell type - Beas-2B

Get tips on using Autophagy Assay Kit to perform Autophagy assay cell type - Mouse cardiomyocytes