DNA transfection Mammalian cells

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using EasySep™ Direct Human T Cell Isolation Kit to perform Cell Isolation Human T cells

Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized MIA PaCa-2

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary lung epithelial cells-organoids

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media Mouse primary mammary ephitelial cells- organoids

Get tips on using StemSpan™ SFEM to perform Stem cell Differentiation media hiPSCs differentiation into mesodermal lineage cells

Get tips on using Minimum Essential Medium Eagle to perform Stem cell Differentiation media hDPSCs differentiation into chondrogenic cells

Get tips on using Minimum Essential Medium Eagle to perform Stem cell Differentiation media hDPSCs differentiation into osteogenic cells

Get tips on using Leukocyte Alkaline Phosphatase Kit to perform Acid phosphatase assay cell type - human periodontal ligament cells