Protein Expression Eukaryotic cells Hi5

Get tips on using Anti-phospho-Histone H2A.X (Ser139) Antibody to perform Immunofluorscence  phospho-Histone H2A.X (Ser139) - Mouse Human

Get tips on using Anti-HIF-1 alpha antibody - ChIP Grade (ab2185) to perform Western blotting HIF-1 alpha

Get tips on using TeSR™-E6 to perform 3D Cell Culture Media hiPSC-derived kidney organoids

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media hiPSC-derived midbrain organoids

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media hiPSC-derived cortical organoids

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media hiPSC-derived forebrain organoids

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Advanced RPMI 1640 Medium to perform 3D Cell Culture Media hiPSC-derived kidney organoids

Get tips on using Gibco™Neurobasal™ Medium to perform 3D Cell Culture Media hiPSC-inner ear organoids

Get tips on using Gibco DMEM/F-12, HEPES to perform 3D Cell Culture Media hiPSC-derived retinal organoids