rna-isolation-purification-cells-primary-rat-brain-microvascular-endothelial-cells

Get tips on using pTip-QC2-gi_21222831 to perform Protein Expression Prokaryotic cells - R. erythropolis putative merR-family transcriptional regulator

Get tips on using pTip-QC2-gi_21222214 to perform Protein Expression Prokaryotic cells - R. erythropolis putative araC-family transcriptional regulator

Get tips on using pTY- α-amylase to perform Protein Expression Prokaryotic cells - E. coli Pyrococcus woesei Hyperthermophile α-Amylase

Get tips on using pST44-yAda3Δ2HIS-yAda2Δ1-yGcn5 to perform Protein Expression Prokaryotic cells - E. coli Ada2/Ada3/Gcn5 complex

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using pET-Sac-Aβ(M1–42) to perform Protein Expression Prokaryotic cells - E. coli Aβ(M1–42)

Get tips on using pET-21b(+)/Pro j 1 to perform Protein Expression Prokaryotic cells - E. coli Pro J 1

Get tips on using His-Strep pQE-TriSystem Vector Set to perform Protein Expression Prokaryotic cells - E. coli Integrin αV

Get tips on using Stealth siRNA(r)_Plat to perform siRNA / miRNA gene silencing Rat - NPC tPA/Plat

Get tips on using Stealth siRNA(r)_BIRC2 to perform siRNA / miRNA gene silencing Rat - B35 cIAP1/BIRC2