siRNA / miRNA gene silencing Rat RMC-1

Get tips on using Human FGF-10 Antibody to perform Immunohistochemistry Human - FGF-10

Get tips on using DMEM/F-12 to perform Mammalian cell culture media MCF-10A

Get tips on using StepUp 100 bp DNA Ladder to perform DNA Ladder 100 bp

Get tips on using Fluorescent 100 bp DNA Ladder to perform DNA Ladder 100 bp

Get tips on using psIL-15Rα/Fc to perform Protein Expression Eukaryotic cells - HEK293 sIL-15Rα/Fc

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using pBESTExp4:IL-10 to perform Protein Expression Prokaryotic cells - B. bifidum murine IL-10

Get tips on using pBESTBL1181:IL-10 to perform Protein Expression Prokaryotic cells - B. bifidum murine IL-10

Get tips on using TriDye™ 100 bp DNA Ladder to perform DNA Ladder 100 bp

Get tips on using TrackIt™ 100 bp DNA Ladder to perform DNA Ladder 100 bp