ChIP H3K36Me3 Bovine Rabbit

Get tips on using LAMP2 (D5C2P) Rabbit mAb to perform Autophagy assay cell type - GH3

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - HaCaT

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - A549

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - HeLa

Get tips on using 53BP1 Antibody (H-300) rabbit polyclonal to perform Immunohistochemistry 53BP1 - Rabbit IgG Human -NA-

Get tips on using Vimentin (D21H3) XP® Rabbit mAb to perform Immunohistochemistry Vimentin - Rabbit Human / mouse -NA-

Get tips on using Anti-LC3B antibody produced in rabbit to perform Western blot LC3B - Rabbit Human -NA-

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Atg5 (D5G3) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y