ChIP H3K4Me3 Mouse

Get tips on using EpiQuik Chromatin Immunoprecipitation (ChIP) Kit to perform ChIP Mouse - Lung

Get tips on using ChIP-IT High Sensitivity® to perform ChIP Mouse - Kidney

Get tips on using EpiQuik Chromatin Immunoprecipitation (ChIP) Kit to perform ChIP Mouse - Kidney

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - Podocyte

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Mouse - Podocyte

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - HSC

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - BMDCs

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Mouse - RAW264.7

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - HT22

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.