ChIP H3K9me3 Mouse

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Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - HSC

Products Merck Millipore Chromatin Immunoprecipitation (ChIP) Assay Kit

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - BMDCs

Products Merck Millipore Chromatin Immunoprecipitation (ChIP) Assay Kit

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Mouse - RAW264.7

Products Thermo Fisher Scientific Pierce™ Agarose ChIP Kit

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - HT22

Products Merck Millipore Chromatin Immunoprecipitation (ChIP) Assay Kit

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Proteins ChIP Anti-bodies H3K4me3

Get tips on using SOLiD™ ChIP-Seq Kit, with ChIP magnet to perform ChIP Human - SH-SY5Y

Products Thermo Fisher Scientific SOLiD™ ChIP-Seq Kit, with ChIP magnet

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Mouse - Cardiac fibroblasts

Products Merck Millipore Chromatin Immunoprecipitation (ChIP) Assay Kit

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product in the experimental, add more DNA to the PCR reaction or increase the number of amplification cycles. Choose an alternate, ChIP-validated antibody if the antibody does not work.

Proteins ChIP Anti-bodies H3K4me2

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Proteins ChIP Anti-bodies H3K4me1

Get tips on using TruSeq ChIP Library Preparation Kit to perform ChIP Mouse - Gonadotrope cell lines

Products Illumina TruSeq ChIP Library Preparation Kit

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