ChIP Human

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - SMMC-7721

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Human - THP-1

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - HEK 293

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - SH-SY5Y

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - MIA PaCa-2

Get tips on using Pierce™ Magnetic ChIP Kit to perform ChIP Human - Fibroblast cell lines

Get tips on using ChIP-IT® Express Shearing Kits to perform ChIP Human - SW480

Get tips on using LowCell# ChIP kit protein A x16 to perform ChIP Human - HepG2

Get tips on using EpiQuik Acetyl-Histone H4 ChIP Kit to perform ChIP Human - HepG2

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.