FACS CD14

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using CD122 antibody | TU27 to perform Flow cytometry Anti-bodies Human - CD122

Get tips on using Anti-CD133 Antibody, clone 13A4 to perform Immunohistochemistry Mouse - CD133

Get tips on using CD151 siRNA (h) to perform siRNA / miRNA gene silencing Human - U251 CD151

Get tips on using Biotin Rat Anti-CD11b to perform Flow cytometry Anti-bodies Mouse - CD11b

Get tips on using Purified Mouse Anti-Human CD135 to perform Flow cytometry Anti-bodies Human - CD135

Get tips on using PE Mouse Anti-Human CD135 to perform Flow cytometry Anti-bodies Human - CD135

Get tips on using PE Mouse Anti-Human CD122 to perform Flow cytometry Anti-bodies Human - CD122

Get tips on using PE Mouse Anti-Human CD116 to perform Flow cytometry Anti-bodies Human - CD116

Get tips on using Purified Mouse Anti-Human CD10 to perform Flow cytometry Anti-bodies Human - CD10