Western blot p62/SQSTM1 Mouse IgG2b

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - CaCo-2

Get tips on using SQSTM1 Antibody (D-3) to perform Autophagy assay cell type - SH-SY5Y

Get tips on using SQSTM1 Antibody (D-3) to perform Cell cytotoxicity / Proliferation assay cell type - K562

Get tips on using SQSTM1 monoclonal antibody (M01), clone 2C11 to perform Autophagy assay cell type - AML12

Get tips on using p62 (human) polyclonal antibody to perform Immunohistochemistry Mouse - p62

Get tips on using IRDye® 800CW Streptavidin to perform Western blot Biotin - -NA- -NA- Infrared dye 800CW

Get tips on using Anti-LC3B antibody produced in rabbit to perform Western blot LC3B - Rabbit Human -NA-

Get tips on using Lectin from Triticum vulgaris (wheat) to perform Western blot 1,4 β-N-acetyl-D-glucosamine - Triticum vulgaris Bacteria Biotin

Get tips on using Goat Anti-Rabbit IgG (H + L)-HRP Conjugate to perform Western blot Secondary Antibody - Goat Rabbit Horseradish peroxidase

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.