CRISPR Mouse Deletion RMA cells

Get tips on using PE Rat Anti-Mouse Ly-6G to perform Flow cytometry Anti-bodies Mouse - Ly6C/Gr-1/Ly6G

Though DNA quantification is but one small step in the multifaceted DNA sample preparation workflow, it can have large implications on the performance and validity of conclusions drawn from downstream assays. Major challenges include accuracy, precision, reproducibility, and detection of present contamination. Among UV spectrophotometry, fluorescence and real-time PCR based methods, the quantification method should be chosen based on the requirement of the downstream assay.

Get tips on using Mouse Sonic Hedgehog/Shh DuoSet ELISA to perform ELISA Mouse - ShhN

Get tips on using Mouse MPO ELISA Kit (Myeloperoxidase) (ab155458) to perform ELISA Mouse - MPO

Get tips on using pro-MMP-9 Mouse ELISA Kit to perform ELISA Mouse - MMP9

Get tips on using Mouse GDNF PicoKine™ ELISA Kit to perform ELISA Mouse - GDNF

Get tips on using Mouse Fibronectin PicoKine™ ELISA Kit to perform ELISA Mouse - Fibronectin

Get tips on using Mouse Decorin ELISA Kit (DCN) (ab155454) to perform ELISA Mouse - Decorin

Get tips on using Mouse BDNF PicoKine™ ELISA Kit to perform ELISA Mouse - BDNF

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.