Protein Expression Prokaryotic cells L. citreum

Get tips on using pBESTExp4:IL-10 to perform Protein Expression Prokaryotic cells - B. bifidum murine IL-10

Get tips on using pBESTBL1181:IL-10 to perform Protein Expression Prokaryotic cells - B. bifidum murine IL-10

Get tips on using pBIN61:α-AIC3 to perform Protein Expression Prokaryotic cells - A. tumefaciens α-amylase inhibitor

Get tips on using MrNV-pGEX-6P-1 to perform Protein Expression Prokaryotic cells - E. coli MrNV capsid

Get tips on using pIRES2-EGFP-PBD-1 to perform Protein Expression Prokaryotic cells - E. coli PBD1-EGFP

Get tips on using pRSET A-FhFtn-1 to perform Protein Expression Prokaryotic cells - E. coli FhFtn-1

Get tips on using pTRAkc-AH/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp

Get tips on using pTRAkc-ERH/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp

Get tips on using DMEM with Glucose and L-Glutamine to perform 3D Cell Culture Media U87MG cells- glioblastoma spheres

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.