FACS CD66b Mouse Human

Get tips on using QIAamp Fast DNA Stool Mini Kit (50) to perform DNA isolation / purification Tissue - fecal sample

Get tips on using EpiTect Fast DNA Bisulfite Kit (50) to perform DNA methylation profiling Gene specific profiling - Hep3B SPRY1

Get tips on using FastDNA™ SPIN Kit for Feces to perform DNA isolation / purification Bacteria - Gram positive Clostridium difficile

Get tips on using Fast MicroSEQ™ D2 Fungal rDNA PCR Kit to perform Cell Culture Contamination Detection Kit Fungi

Get tips on using Type-it Fast SNP Probe PCR Kit (4000) to perform Cell line authentication Peripheral blood lymphocytes

Get tips on using LightCycler® FastStart DNA Master SYBR Green I to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG to perform PCR Multiplex PCR - Mammalian DNA

DNA ladder is typically used as a reference to estimate the size of unknown DNA samples that are separated based on their mobility in an electrical field. The critical points for running a DNA ladder are compatibility with running buffer, agarose gel percentage, and choosing the correct range of DNA ladder for sizing DNA molecules.

Get tips on using Silencer®_Faslg siRNA (r) to perform siRNA / miRNA gene silencing Rat - F98 Faslg

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.