Site Directed Mutagenesis (SDM) Human Point mutation MDA-MB-231

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using Silencer® Select_FPr1/Silencer® Select_FPr2 siRNA to perform siRNA / miRNA gene silencing Mouse - Neuro 2a Fpr1/Fpr2

Get tips on using QIAGEN Plasmid Kits to perform Plasmid Isolation Stenotrophomonas maltophilia

Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media Murine mammospheres

Get tips on using MagAttract PowerSoil DNA KF Kit to perform DNA isolation / purification Tissue - fecal sample

Get tips on using MagAttract PowerMicrobiome DNA/RNA Kit to perform DNA isolation / purification Micorbiome - Fecal sample

Get tips on using MagAttract PowerSoil DNA EP Kit to perform DNA isolation / purification Micorbiome - Fecal sample