Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using Gibco™ Advanced DMEM to perform Stem cell culture media Ovarian Stem Cells
Get tips on using Atg12 (D88H11) Rabbit mAb to perform Autophagy assay cell type - RPE-J cells
Get tips on using ATG5 Antibody (N-term) to perform Autophagy assay cell type - RPE-J cells
Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media hMegakaryoblastic cells
Get tips on using Gibco™ RPMI 1640 Medium to perform Stem cell culture media hMegakaryoblastic cells
Get tips on using Gibco™ DMEM, high glucose to perform Stem cell culture media hMyoblasts cells
Get tips on using Gibco™DMEM/F-12 to perform Stem cell culture media hMyoblasts cells
Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Paneth cells
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment