DNA isolation / purification Cells Primary cells

Get tips on using MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging to perform ROS assay cell type - A549 human adenocarcinomic human alveolar basal epithelial cells

Get tips on using QIAshredder (250) to perform DNA fragmentation Cell lines

Get tips on using Comet Assay to perform DNA Damage Assay A-375

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform DNA Damage Assay Saos-2

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.

Get tips on using Corning® 500 mL SF Medium, [+] L-glutamine and 1 g/L BSA to perform Stem cell culture media Ovarian cancer stem cells (Caov3, 3AO, SKOV3)

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay MCF7

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay HT1080

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay HeLa

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay A549