Site Directed Mutagenesis (SDM) Mouse L929

Get tips on using Anti-SMAD1/5/9 antibody (ab66737) to perform Western blotting Smad1

Get tips on using Smad1 Antibody (A-4): sc-7965 to perform Western blotting Smad1

Get tips on using Recombinant Anti-Smad4 antibody [EP618Y] (ab40759) to perform Western blotting Smad4

Get tips on using Smad4 Antibody (B-8): sc-7966 to perform Western blotting Smad4

Get tips on using Smad2 Antibody (YZ-13): sc-101153 to perform Western blotting Smad2

Get tips on using Anti-Smad2 (phospho S467) antibody (ab53100) to perform Western blotting Smad2

Get tips on using Phospho-SMAD2 (Ser465, Ser467) Monoclonal Antibody (H.205.4) to perform Western blotting Smad2

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using Recombinant Anti-alpha smooth muscle Actin antibody [E184] (ab32575) to perform Western blotting Smooth muscle actin

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.