Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP) Mouse

Get tips on using Anti-Glucocorticoid Receptor antibody (ab3578) to perform Immunohistochemistry Human - GR/glucocorticoid receptor

Get tips on using Recombinant Anti-MUC1 antibody [EPR1023] (ab109185) to perform Immunohistochemistry Human - Muc-1

Get tips on using Mouse GFR alpha-3/GDNF R alpha-3 Antibody to perform Immunohistochemistry Mouse - Gfrα3

Get tips on using Anti-Dicer antibody [13D6] - ChIP Grade (ab14601) to perform Immunohistochemistry Human - Dicer1

Get tips on using Anti-Androgen Receptor antibody [AR 441] (ab9474) to perform Immunohistochemistry Human - AR

Get tips on using Anti-Villin antibody [3E5G11] - N-terminal (ab201989) to perform Immunohistochemistry Human - Villin

Get tips on using Anti-Glutamine Synthetase Antibody, clone GS-6 to perform Immunohistochemistry Rat - GS

Get tips on using Human/Mouse GFR alpha-2/GDNF R alpha-2 Antibody to perform Immunohistochemistry Mouse - Gfrα2

Get tips on using Anti-pan Cytokeratin antibody [C-11] (ab7753) to perform Immunohistochemistry Human - pan Cytokeratin

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.