Protein expression and purification Mammalian cells HEK 293

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Human Heart

Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Tissue - heart

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

Get tips on using TRI Reagent® MRC to perform RNA isolation / purification Tissue - rat heart tissue

Get tips on using PAXgene Tissue RNA/miRNA Kit to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Heart

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Heart