Protein expression and purification Mammalian cells HEK 293

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Human Heart

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Bacteria - Gram negative Helicobacter pylori

Get tips on using PAXgene Tissue miRNA Kit to perform RNA isolation / purification Tissue - rat heart muscle tissue

Get tips on using QIAamp DNA Micro Kit to perform DNA isolation / purification Bacteria - Gram negative Helicobacter pylori

Get tips on using Qproteome FFPE Tissue Kit (20) to perform Protein isolation Tissue - Human tissue C-MFPE samples

Get tips on using RNeasy Lipid Tissue Mini Kit to perform RNA isolation / purification Tissue - Mouse Cerebral hemispheres

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Bacteria - Gram negative Helicobacter pylori

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.