rna-isolation-purification-cells-primary-mouse-cortical-neurons

Get tips on using NF-κB p65 (D14E12) XP® Rabbit mAb #8242 to perform Immunohistochemistry Mouse - NFκB / p65

Get tips on using OSTEOPONTIN (O-17) ANTI-HUMAN RABBIT IGG AFFINITY PURIFY to perform Immunohistochemistry Mouse - Spp1/OPN

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Rabbit eye retina/choroids

RNAi or RNA interference is a common method to suppress gene expression in vitro/in vivo by utilizing the inherent microRNA machinery, without introducing a total gene knockout. miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid-mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time-consuming, but provide a more permanent expression of RNAi in the cells and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines.

Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - mouse 3T3-L1

Get tips on using MouseTRAP™ (TRAcP 5b) ELISA to perform Acid phosphatase assay cell type - murine macrophage cells

Get tips on using CelLytic™ B Cell Lysis Reagent to perform Protein isolation Bacteria - Bacillus cellulosilyticus

Get tips on using Anti-Beclin 1 (Human) pAb to perform Autophagy assay cell type - Mouse white adipose tissue

Get tips on using Anti-SQSTM1 / p62 antibody (ab56416) to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Anti-p62 (SQSTM1) (Human) pAb to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)