DNA isolation / purification Cells

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - RAW264.7

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - HeLa

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - SiHa

Get tips on using RealTime-Glo™ MT Cell Viability Assay to perform Live / Dead assay mammalian cells - INS-1 832/12

Get tips on using In Situ Cell Death Detection Kit, TMR red to perform TUNEL assay cell type - HEK293 human embryonic kidney cells

Get tips on using MTT Assay solution to perform Cell cytotoxicity / Proliferation assay cell type - adipose stem cells

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Microglia Medium to perform Stem cell Differentiation media hiPSCs differentiation into Microglial-like cells

Get tips on using DMEM/F-12 to perform 3D Cell Culture Media PLC/PRF/5 cells-spheres